蛋白凝胶电泳及相关的Western-Blot是蛋白分析的主流技术 (Figure 1)。GeneCopoeia公司提供两种蛋白染料： 1) Coomassie® Blue Fast Stain, 一种即用型的蛋白染料，无需甲醇或乙酸固定、或脱色； 2) eLuminol™ Protein Gel Stain，一种具有极高灵敏度的蛋白荧光染料，用于检测SDS-PAGE凝胶电泳中的总蛋白。GeneCopoeia提供的 ECL Western Blotting Detection Kit用于WB检测HRP标记的抗体及其关联的抗原，具有快速检测、灵敏度极高等特点。另外，GeneCopoeia 也提供了两种蛋白定量分析试剂盒： 1) Bradford Protein Assay Kit；和 2) BCA Protein Assay Kit。
Figure 1. Protein gel analysis workflow
蛋白染料选购指南
| Coomassie® Blue Fast Stain | eLuminol™ Protein Gel Stain | |
| 灵敏度 | 20 ng | 0.5 ng |
| 操作性 | Ready to use | Supplied as stock solution |
| 耗时 | 2 hr (standard) 20 min (microwave) |
90 min (standard) 30 min (microwave) |
| 检测方法 | 比色法 | 荧光 |
| 质谱兼容 | 是 | 是 |
 |
Figure 2. Gel stain results with Coomassie® Blue Fast Stain (left), eLuminol™ Protein Gel Stain (middle), followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.
| ECL化学发光检测试剂盒 |
Figure 3. Detection of protein in Western Blots with ABP kit (left) and Millipore Kit (right).
| Bradford Protein Assay Kit | BCA Protein Assay Kit |
Figure 4. BSA standard curve with Bradford Protein Assay Kit (left) and BCA Protein Assay Kit (right).
