Mouse Monoclonal IgG2a
Catalog Number
CGAB-GST-0100 (100 µg)
CGAB-GST-0050 (50 µg)
Description
Anti-GST (glutathione-S-transferase) tag antibody is a purified mouse IgG2a monoclonal antibody that binds to GST-fusion proteins. Anti-GST tag antibody can be used to identify and capture fusion proteins by western blots and immunoprecipita-tion. Immobilized anti-GST is also used for affinity purification of GST fusion proteins. OmicsLink™ anti-tag antibodies are ideal for use with OmicsLink™ expression-ready ORF cDNA clones.
Immunogen
The OmicsLink™ anti-GST tag antibody was produced by immunizing mice with a synthetic peptide of Schistosoma japonicum GST.
Specificity
The anti-GST tag monoclonal antibody detects over-expressed GST fusion proteins.
 |
Figure 1. 20 ng of recombinant GST protein (lane 1) and 20 µg of 293 cell lysates were transfected with a GST expression vector and probed with OmicsLink Anti-GST (1 µg/ml). |
Formulation
0.25 mg/ml in PBS, pH 7.4 with 0.05% sodium azide and 50% (v/v) glycerol.
Applications
Western blot, immunoprecipitation, immunostaining and affinity purification.
Storage/Stability
Stable for 1 year at -20°C from shipment date. Briefly centrifuge the original vial prior to opening the cap to collect the product at the bottom of the tube.
Starting dilution recommendations
Western blot at 1:1000
immunoprecipitation and immunostaining at 1:200
| To order | Product Sheet |
